Confirmation of Connexin45 Underlying Weak Gap Junctional Intercellular Coupling in HeLa Cells
Gap junctions (GJs) are intercellular channels that connect adjacent cells electrically and metabolically.The iodide-yellow fluorescent protein (I-YFP) gap junctional intercellular communication (GJIC) assay is a recently developed method with high sensitivity.HeLa cells have been widely used as GJ-deficient cells for GJ-related research.Herein, we